Laboratory-Scale Evaluation of Indigenous Extracellular Enzyme-Producing Bacteria for Wastewater Quality Improvement

Rocznik Ochrona Środowiska 2026, vol. 28, pp. 688-696

Duha Yousif Aziz , Aalaa Fahim Abbas This email address is being protected from spambots. You need JavaScript enabled to view it.

	University of Al-Qadisiyah, Iraq
	This email address is being protected from spambots. You need JavaScript enabled to view it.
	https://doi.org/10.54740/ros.2026.046

This study evaluated indigenous bacteria isolated from sludge for extracellular enzyme production and assessed their potential at the laboratory scale to improve wastewater quality. Forty sludge samples were diluted in a series and spread onto nutrient agar plates. Single bacterial colonies were subsequently screened on wastewater agar medium, and seven growing bacterial isolates were identified by 16S rRNA gene sequencing; their sequences were deposited in GenBank. Amylase, lipase, and protease activities were assessed, and five representative isolates were tested in non-sterile wastewater batch cultures for 72 h. Wastewater quality parameters were analyzed using one-way analysis of variance and Tukey's test. The isolates showed variation in enzymatic activity and treatment efficiency. Enterococcus faecalis DAQS7 achieved the highest overall removals of chemical oxygen demand, biochemical oxygen demand, phosphate, and nitrate, at 86.57%, 90.00%, 31.25%, and 68.66%, respectively. Staphylococcus aureus DAQSE3 and Enterobacter cloacae DAQSE5 achieved the highest total suspended solids removal with 90.12% and 88.07%, respectively.

bioaugmentation, 16S rRNA, extracellular enzymes, indigenous bacteria, municipal wastewater

AMA Style
Aziz D.Y., Abbas A.F. Laboratory-Scale Evaluation of Indigenous Extracellular Enzyme-Producing Bacteria for Wastewater Quality Improvement. Rocznik Ochrona Środowiska. 2026; 28. https://doi.org/10.54740/ros.2026.046

ACM Style
Aziz D.Y., Abbas A.F. 2026. Laboratory-Scale Evaluation of Indigenous Extracellular Enzyme-Producing Bacteria for Wastewater Quality Improvement. Rocznik Ochrona Środowiska. 28. DOI:https://doi.org/10.54740/ros.2026.046

ACS Style
Aziz D.Y.; Abbas A.F. Laboratory-Scale Evaluation of Indigenous Extracellular Enzyme-Producing Bacteria for Wastewater Quality Improvement. Rocznik Ochrona Środowiska 2026, 28, 688-696. https://doi.org/10.54740/ros.2026.046

APA Style
Aziz D.Y., & Abbas A.F. (2026). Laboratory-Scale Evaluation of Indigenous Extracellular Enzyme-Producing Bacteria for Wastewater Quality Improvement. Rocznik Ochrona Środowiska, 28, 688-696. https://doi.org/10.54740/ros.2026.046

ABNT Style
AZIZ D.Y.; ABBAS A.F. Laboratory-Scale Evaluation of Indigenous Extracellular Enzyme-Producing Bacteria for Wastewater Quality Improvement. Rocznik Ochrona Środowiska, v. 28, p. 688-696, 2026. https://doi.org/10.54740/ros.2026.046

Chicago Style
Duha Yousif Aziz and Aalaa Fahim Abbas. 2026. "Laboratory-Scale Evaluation of Indigenous Extracellular Enzyme-Producing Bacteria for Wastewater Quality Improvement". Rocznik Ochrona Środowiska 28, 688-696. https://doi.org/10.54740/ros.2026.046

Harvard Style
Aziz D.Y. and Abbas A.F. (2026) "Laboratory-Scale Evaluation of Indigenous Extracellular Enzyme-Producing Bacteria for Wastewater Quality Improvement", Rocznik Ochrona Środowiska, 28, pp. 688-696. doi:https://doi.org/10.54740/ros.2026.046

IEEE Style
D. Y. Aziz and A. F. Abbas, "Laboratory-Scale Evaluation of Indigenous Extracellular Enzyme-Producing Bacteria for Wastewater Quality Improvement", RoczOchrSrod, vol. 28, pp. 688-696. https://doi.org/10.54740/ros.2026.046