The Use of Giant Miscanthus (Miscanthus × Giganteus) in 2G Bioethanol Production

Rocznik Ochrona Środowiska 2023, vol. 25, pp. 413-422

Małgorzata Smuga-Kogut This email address is being protected from spambots. You need JavaScript enabled to view it., Sławomir Bugajski, Tomasz Piskier , Bartosz Walendzik This email address is being protected from spambots. You need JavaScript enabled to view it.

	Koszalin University of Technology, Poland
	This email address is being protected from spambots. You need JavaScript enabled to view it. This email address is being protected from spambots. You need JavaScript enabled to view it.
	https://doi.org/10.54740/ros.2023.042

The aim of the study was to conduct the process of bioethanol obtaining from biomass using chemical treatment and enzymatic hydrolysis. Different concentrations of sodium hydroxide (5 and 10%) were used for the delignification process, and enzymatic hydrolysis was carried out using three commercial cellulolytic preparations (Cellic® CTec2, cellulase from Trichoderma reesei and cellulase from Aspergillus species). The final step involved an alcoholic fermentation process using Saccharomyces cerevisiae TYPE II yeast. After enzymatic hydrolysis, the content of reducing sugars was determined in the samples, and the fermentation yield was controlled by determining the ethanol content by pycnometry. The use of chemical pretreatment increased the yield of the whole process by at least 50%. The content of reducing sugars after hydrolysis depended on the type of enzyme preparation used for hydrolysis and the use of NaOH in pretreatment. The highest content of reducing sugars (45.8 g/dm³) was achieved in a sample of material that was purified with 5% NaOH, and enzymatic hydrolysis was carried out using Cellic® CTec2. This means an efficiency of the enzymatic hydrolysis process equal to 94.69%. The concentration of bioethanol after alcoholic fermentation in this sample was 0.509 g/l.

giant miscanthus, bioethanol, chemical treatment of biomass, enzymatic hydrolysis, alcoholic fermentation

AMA Style
Smuga-Kogut M, Bugajski S, Piskier T, Walendzik B. The Use of Giant Miscanthus (Miscanthus × Giganteus) in 2G Bioethanol Production. Rocznik Ochrona Środowiska. 2023; 25. https://doi.org/10.54740/ros.2023.042

ACM Style
Smuga-Kogut, M., Bugajski, S., Piskier, T., Walendzik, B. 2023. The Use of Giant Miscanthus (Miscanthus × Giganteus) in 2G Bioethanol Production. Rocznik Ochrona Środowiska. 25. DOI:https://doi.org/10.54740/ros.2023.042

ACS Style
Smuga-Kogut, M.; Bugajski, S.; Piskier, T.; Walendzik, B. The Use of Giant Miscanthus (Miscanthus × Giganteus) in 2G Bioethanol Production Rocznik Ochrona Środowiska 2023, 25, 413-422. https://doi.org/10.54740/ros.2023.042

APA Style
Smuga-Kogut, M., Bugajski, S., Piskier, T., Walendzik, B. (2023). The Use of Giant Miscanthus (Miscanthus × Giganteus) in 2G Bioethanol Production. Rocznik Ochrona Środowiska, 25, 413-422. https://doi.org/10.54740/ros.2023.042

ABNT Style
SMUGA-KOGUT, M.; BUGAJSKI, S.; PISKIER, T.; WALENDZIK, B. The Use of Giant Miscanthus (Miscanthus × Giganteus) in 2G Bioethanol Production. Rocznik Ochrona Środowiska, v. 25, p. 413-422, 2023. https://doi.org/10.54740/ros.2023.042

Chicago Style
Smuga-Kogut, Małgorzata, Bugajski, Sławomir, Piskier, Tomasz, Walendzik, Bartosz. 2023. "The Use of Giant Miscanthus (Miscanthus × Giganteus) in 2G Bioethanol Production". Rocznik Ochrona Środowiska 25, 413-422. https://doi.org/10.54740/ros.2023.042

Harvard Style
Smuga-Kogut, M., Bugajski, S., Piskier, T., Walendzik, B. (2023) "The Use of Giant Miscanthus (Miscanthus × Giganteus) in 2G Bioethanol Production", Rocznik Ochrona Środowiska, 25, pp. 413-422. doi:https://doi.org/10.54740/ros.2023.042

IEEE Style
M. Smuga-Kogut, S. Bugajski, T. Piskier, B. Walendzik, "The Use of Giant Miscanthus (Miscanthus × Giganteus) in 2G Bioethanol Production", RoczOchrSrod, vol 25, pp. 413-422. https://doi.org/10.54740/ros.2023.042